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KMID : 0381120070290030315
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Genes and Genomics
2007 Volume.29 No. 3 p.315 ~ p.322
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Genetic Diversity of Tunisian Local Barley Accessions Analyzed with Morphological and RAPD Makers: Relationship between the Two Methods
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Raoudha Abdellaoui
Mustapha Rouaissi
Synda Chenennaoui
M’barek Ben Naceur
Jeannette Ben Hmida
Bettaib Ben Kaab Leila
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Abstract
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Thirteen Tunisian barley (Hordeum vulgare L.) accessions were collected from different regions. Morphological traits were studied and analyzed using principal components analysis (PCA) and clusters were constrained based on median joining distance. Further, genetic diversity was studied using RAPD markers. The cultivate variety ‘Martin’ was added in these essays. Both methods were used to compare how morphological traits and RAPD molecular markers described accessions relationship. Both analysis results showed a high degree of variation among analyzed accessions, indicating an important source of genetic diversity that can be used in future breeding programs. Morphological PCA traits and cluster indicated geographic information. Indeed, they grouped barley accessions according to genetic criteria such as ear attitude, length of glum and its awn relative to grain. Distances obtained by each of the approaches were compared with special attention to the coincidences and divergences between the two methods. Comparison of morphological and molecular data using the Mantel test indicated a very low correlation (r = 0.12). Therefore, both techniques are not complementary but necessary for barley characterization.
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KEYWORD
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Barley accessions, Mantel test, morphological traits, RAPD markers, genetic diversity
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